Journal: International Journal of Molecular Sciences

Article Title: LPPR5 Expression in Glioma Affects Growth, Vascular Architecture, and Sunitinib Resistance

doi: 10.3390/ijms23063108

Figure Lengend Snippet: In-silico analysis of LPPR5 in physiological brain tissue and primary brain tumor. ( a ) LPPR5 expression is physiologically high in human adult brain. Outside the CNS, LPPR5 is compara-tively highly expressed in testis [GTeX gene expression data for LPPR5 in brain (blue, Hippocampus light green) and non-brain tissue (dark green). Expression values are shown in TPM (Tran-scripts Per Million), calculated from a gene model with isoforms collapsed to a single gene. No other normalization steps have been applied. Box plots are shown as median and 25th and 75th per-centiles; points are displayed as outliers if they are above or below 1.5 times the interquartile range]. ( b ) LPPR5 is significantly un-der-expressed in (high-grade) glioma. Relative under-expression rank is median rank across all analyses in gene expression in Sun Brain, and respectively Roth normal in Oncomine [p for medi-an-ranked analysis, Oncomine visualization and statistical anal-ysis]. ( c ) A subgroup of high-grade glioma has decreased LPPR5 expression; in lower grade glioma, the ratio of tumors with low LPPR5 expression is smaller [mRNA expression data from Sun Brain, each bar represents a single tumor, II and III indicates astrocytoma WHO grade]. ( d ) On the DNA-level, LPPR5 is deleted in most oligodendrogliomas and to a lesser degree in lower grade astrocytic gliomas and GBM [Oncomine visualization of LPPR5 DNA copy number units of the tumors in Kotliarov Brain, each bar represents a single tumor]. ( e ) LPPR5 expression is significantly lower in the mesenchymal expression subtype of GBM ( n = 49) compared to classical ( n = 39, * p = 0.0451) and proneural ( n = 29, **** p < 0.0001) subtypes. Expression in the neural subtype ( n = 26) is significantly lower than in proneural subtype (* p = 0.0162) [TCGA via cBio]. ( f ) Histogram plot of 70 human Glioma cell lines screened, 50 show low expression of LPPR5 [Cancer Cell Line Encyclopedia, Broad Institute].

Article Snippet: Moreover, in a screening of 70 human glioma cell lines from the Cancer Cell Line Encyclopedia (Broad Institute), only 4 cell lines showed a significant expression of LPPR5 (LN319, KNS42, TM31, and NMCG1; f).

Techniques: In Silico, Expressing, Gene Expression

Journal: Cancer Management and Research

Article Title: The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy

doi: 10.2147/CMAR.S463694

Figure Lengend Snippet: EMILIN3 gene function experiment in LN229. qRT-PCR transfected LN229 human glioma cells efficiently (A) . The CCK-8 experiment and live/dead staining findings demonstrated that overexpression of the EMILIN3 gene may increase LN229 cell line proliferation and vitality ( B and C ). Overexpression of the EMILIN3 gene enhances the migration, invasiveness, and colony formation of the LN229 cell line (D-F ).

Article Snippet: The LN229 and HS-683 human glioma cell line were purchased from iCell Bioscience Inc, and grown in DMEM media (Gibco, USA) with 10% foetal bovine serum (BI, USA) and 1g/mL penicillin/streptomycin (Hyclone, USA) at 37 degrees Celsius and 5% carbon dioxide.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Over Expression, Migration

Journal: Frontiers in Oncology

Article Title: System analysis based on the migration- and invasion-related gene sets identifies the infiltration-related genes of glioma

doi: 10.3389/fonc.2023.1075716

Figure Lengend Snippet: EMP3 silencing attenuates the migration and invasion of glioma cells. (A) The expression of EMP3 in different cell lines in the CCLE datasets. (B–D) Western blot and qPCR display EMP3 express highly in U118 and A172. (E–G) The growth curve and plate cloning experiment display that EMP3 silencing has little effect on the cell proliferation of U118 and A172. (H, I) EMP3 knockdown inhibits the migration and invasion of glioma cells. ( J–O , 20×) qPCR displays EMP3 silencing attenuates the expression of MMP2 and MMP9 expression (ns, p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: The normal human astroglia (NHA), which were purchased from the Chongqing Golden Magpie Technology Development Co., and U251, LN229, A172, U118, and U87 cells, which were purchased from the China Center for Type Culture Collection (Shanghai, China), were cultured in the DMEM medium supplemented with 10% FBS (HyClone, UT, USA) and 1% penicillin–streptomycin (Beyotime Biotechnology, Jiangsu, China).

Techniques: Migration, Expressing, Western Blot, Cloning, Knockdown

Journal: Frontiers in Oncology

Article Title: System analysis based on the migration- and invasion-related gene sets identifies the infiltration-related genes of glioma

doi: 10.3389/fonc.2023.1075716

Figure Lengend Snippet: EMP3 silencing suppressed EMT marker expression. (A) Western blot analysis of U118 transfected with indicated siRNAs targeting EMP3 (siEMP3#1, siEMP3#2, and siEMP3#3) or siNC. (B) Western blot analysis of A172 transfected with indicated siRNAs targeting EMP3 (siEMP3#1, siEMP3#2, and siEMP3#3) or siNC. (p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

Article Snippet: The normal human astroglia (NHA), which were purchased from the Chongqing Golden Magpie Technology Development Co., and U251, LN229, A172, U118, and U87 cells, which were purchased from the China Center for Type Culture Collection (Shanghai, China), were cultured in the DMEM medium supplemented with 10% FBS (HyClone, UT, USA) and 1% penicillin–streptomycin (Beyotime Biotechnology, Jiangsu, China).

Techniques: Marker, Expressing, Western Blot, Transfection